Artificial intelligence (AI) and machine learning (ML) are advancing rapidly and in use across industries, including biomedical research and healthcare delivery. For this full-day public workshop, NIH brought together leaders in innovation and science to explore the opportunities for AI and ML to accelerate medical advances from biomedical research. Workshop participants heard from leading industry experts and scientists who are employing AI/ML in biomedical research settings. Speakers covered a range of issues including the promise of integrating AI technology into healthcare, how it is being used in biomedical research, its potential for enhancing clinical care and scientific discovery. Craig Mundie, who served on the President’s Council of Advisors on Science and Technology (PCAST) and was formerly Microsoft’s Chief Research Strategy Officer, delivered the keynote address.